Glomerular endothelial cells synthesize collagens but little gelatinase A and B.
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چکیده
منابع مشابه
Endothelial cells synthesize and process apolipoprotein B.
We reported previously that a 116-kDa lipoprotein lipase (LPL)-binding protein from endothelial cells has sequence homology to the amino-terminal region of apolipoprotein (apo) B. We now tested whether endothelial cells synthesize apoB mRNA and protein. Primers were designed to the human apoB cDNA sequence and reverse transcription polymerase chain reaction was performed using total RNA isolate...
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Human umbilical vein endothelial cells were analyzed for the presence of prothrombin. factor VII, protein C, and protein S in culture supernatants and cell extracts using specific radioimmunoassays. Only protein S was detected. Conditioned medium from 24-hour cultures and cell lysates contained 21.7 ng/mL and 88.8 ng/107 cells of protein S, respectively. Intrinsic labeling and immunoprecipitati...
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PURPOSE To investigate the capacity of cultured Müller cells to synthesize collagens, since previous studies indicated that Müller cells could be involved in collagen remodeling at the vitreoretinal border in adult human eyes. METHODS Spontaneously immortalized cultured human Müller cells were analyzed for the presence of mRNA of types I-VII, IX, XI, and XVII collagen by RT-PCR. Furthermore, ...
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Protein Z (PZ) is a vitamin K-dependent protein isolated from human plasma, and acts as a cofactor for a serpin, called protein Z-dependent protease inhibitor (ZPI). A prothrombotic phenotype has been reported in PZ deficient mice, and PZ deficiencies have been observed in patients with arterial thrombotic events. PZ was immunologically detected in the endothelium of atherosclerotic arteries, s...
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Cellular fibronectin (Fn) bearing an alternatively spliced extra type 111 structural repeat (ED1 1 is normally present at low concentrations in blood plasma. The source of this material remains uncertain. In this study, primary cultures of human umbilical vein endothelial cells (HUVEC) labeled with 36S-methionine were observed to synthesize Fn monomers both with and without this segment. Monome...
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ژورنال
عنوان ژورنال: Journal of the American Society of Nephrology
سال: 1998
ISSN: 1046-6673,1533-3450
DOI: 10.1681/asn.v9112040